The magic formula I used for calculating percent starch was:
% Starch = [(∆Atest )(900) ]/ [(∆Astd)(sample weight in mg)]
(I won't bore you with details on where the 900 came from but it involves the multiplication of molecular weight of starch monomers, initial sample volumes from glucose sample volume assays, dilution factors, and whatnot.)
Okay. The values for the 1 mg sample, 5 mg sample, and 10 mg sample turned out to be 24.3%, 33.7%, and 56.7%.
Plotted on a graph, the relationship turns out to be somewhat linear, which is a good and expected:
Next week I will repeat the standardization experiment to make sure everything is correct (and to make sure it is repeatable), before moving on to the real samples.
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