This Calls for a New Game Plan

To combat bacterial and fungal contamination, we were going to make the following changes:

1. Use giant cotton balls to stuff the flask openings instead of tinfoil (Dr. B said when she did research in her lab, this method worked quite well.)
2. Autoclave the media longer, with emphasis on listening for a very high whistle
3. Most important change: Sterilize the plants before transferring them to the media to grow

On November 18, I redid the autoclaving procedure:

8:18: started heating the pressure cooker
8:33: faint gas noise readily heard, first signs of bubbles foaming at the valve
8:37: started whistling
8:42: louder, but still no "chugging"
8:43: shut heat off
*Here I had to dash off to Multi class

10:01: turned heat on again
10:30-10:50: let it run